Development of an ImmunoFET for Analysis of Tumour Necrosis Factor-? in Artificial Saliva: Application for Heart Failure Monitoring

Assessing tumour necrosis factor-? (TNF-?) levels in the human body has become an essential tool to recognize heart failure (HF). In this work, label-free, rapid, easy use ImmunoFET based on ion-sensitive field effect transistor (ISFET) was developed for detection of TNF-? protein. Monoclonal anti-TNF-? antibodies (anti-TNF-? mAb) were immobilized ISFET gate made silicon nitride (Si3N4) after salinization with 11-(triethoxysilyl) undecanal (TESUD). The obtained functionalized mAbs (ImmunoFET) used detect protein both phosphate buffer saline (PBS) and artificial saliva (AS). change threshold voltage (?VT) showed approximately linear dependency concentration antigens range 5–20 pg/mL matrixes. cross-selectivity study that demonstrated be selective towards TNF-?, when compared other HF biomarkers such as N-terminal pro-brain natriuretic peptide (NT-proBNP), interleukin-10 (IL-10), cortisol, even if further experiments have carried out decreasing possible unspecific absorption phenomena. To best our knowledge, is first been Si3N4 AS by electrical measurement.